Shuttle vectors
| UID | Plasmid name | Antibiotic resistance | Plasmid Size | Features | Sequence | Primary reference | Notes |
|---|---|---|---|---|---|---|---|
| CT301 | pGFP-SW2 | Chloramphenicol, Ampicillin | 11,539bp |  |
Download sequence | Wang et al., 2011 | SW2 plasmid inserted into pSP73 vector. Carries red-shifted GFP (RSGFP) gene. |
| CT302 | pCDS5KO | Chloramphenicol, Ampicillin | 10,919bp |  |
Download sequence | Wang et al., 2013 | pGFP-SW2 with CDS5 deleted. |
| CT303 | placZ-CDS5KO | Chloramphenicol, Ampicillin | 14,774 bp |  |
Download sequence | Wang et al., 2013 | pCDS5KO with the lacZ gene inserted into the deletion site of CDS5 |
Shuttle vectors are provided as 2µg of purified DNA. These may also be purchased transformed into chlamydia strains L2P- or SWFP-; see below. To order, click on the "order here" button in the left panel, or click here
☆ Please note: Charges for plasmids and transformants are not for profit, but are to cover production costs and shipping. Additionally, these are genetically modified organisms, so please ensure that your organisation has the necessary regulatory permissions to accept these.
Transformed C. trachomatis
| UID | Chlamydia strain | Plasmid name | Primary reference | Notes |
|---|---|---|---|---|
| CT401 | L2P- (CT109) | pSW2-GFP | Wang et al., 2011 | SW2 plasmid inserted into pSP73 vector. Carries red-shifted GFP (RSGFP) gene. See above table for more information on pSW2-GFP. |
| CT402 | SWFP- (CT107) | pSW2-GFP | Wang et al., 2013 | SW2 plasmid inserted into pSP73 vector. Carries red-shifted GFP (RSGFP) gene. See above table for more information on pSW2-GFP. |
| CT403 | A2497 (CT101) | pSW2-GFP | Unpublished | SW2 plasmid inserted into pSP73 vector. Carries red-shifted GFP (RSGFP) gene. A2497 cured of its endogenous plasmid in Southampton, using Novobiocin (as described by O'Connell et al., 2006) prior to transforming with the shuttle vector. See above table for more information on pSW2-GFP. |